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Journal of Applied Genetics 51(3), 2010, pp. 395-402

Bacterial diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16S rDNA analysis

P. R. Pandya, K. M. Singh, S. Parnerkar, A. K. Tripathi, H. H. Mehta, D. N. Rank, R. K. Kothari, C. G. Joshi


Abstract: Bacterial communities in buffalo rumen were characterized using a culture-independent approach for a pooled sample of rumen fluid from 3 adult Surti buffaloes. Buffalo rumen is likely to include species of various bacterial phyla, so 16S rDNA sequences were amplified and cloned from the sample. A total of 191 clones were sequenced and similarities to known 16S rDNA sequences were examined. About 62.82% sequences (120 clones) had >90% similarity to the 16S rDNA database sequences. Furthermore, about 34.03% of the sequences (65 clones) were 85-89% similar to 16S rDNA database sequences. For the remaining 3.14%, the similarity was lower than 85%. Phylogenetic analyses were also used to infer the makeup of bacterial communities in the rumen of Surti buffalo. As a result, we distinguished 42 operational taxonomic units (OTUs) based on unique 16S r DNA sequences: 19 OTUs affiliated to an unidentified group (45.23% of total OTUs), 11 OTUs of the phylum Firmicutes, also known as the low G+C group (26.19%), 7 OTUs of the Cytophaga-Flexibacter-Bacteroides phylum (16.66%), 4 OTUs of Spirochaetes (9.52%), and 1 OTU of Actinobacteria (2.38%). These include 10 single-clone OTUs, so Good’s coverage (94.76%) of 16S rRNA libraries indicated that sequences identified in the libraries represent the majority of bacterial diversity present in rumen.

Key words: Bubalus bubalis, molecular diversity, rumen bacteria, Surti buffalo, 16S rDNA, 16S rRNA.

Correspondence: P. R. Pandya, Ass. Research Scientist (ANRS), College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388 001 Gujarat, India; e-mail: kmsingh18@gmail.com

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