Journal of Applied Genetics 51(3), 2010, pp. 283-287

Mapping of sequence-specific markers and loci controlling preharvest sprouting and alpha-amylase activity in rye (Secale cereale L.) on the genetic map of an F₂ (S120×S76) population

B. Myskow, S. Stojalowski, P. Milczarski, P. Masojc

Abstract: Location of the loci that control preharvest sprouting and alpha-amylase activity in rye was studied based on intercross S120×S76, consisting of 110 genotypes of F₂ and F₃ progenies. The genetic map currently consists of 141 loci distributed in 11 linkage groups, covering a distance of 506.4 cM, and was enriched during this study with 24 sequence-specific markers (7 SCARs, 7 SSRs, and 10 STSs). The extended map was applied for composite interval mapping of the loci controlling preharvest sprouting and α-amylase activity, revealing 3 significant QTLs for preharvest sprouting, located on chromosomes 3R, 5R and 6R (in 1999), and one QTL for α-amylase activity found on chromosome 2R (in 2000).

Key words: alpha-amylase, genetic map, molecular markers, preharvest sprouting, QTL, rye

Correspondence: B. Myskow, Department of Plant Genetics, Breeding and Biotechnology, West-Pomeranian University of Technology, Szczecin, Poland; e-mail: bmyskow@zut.edu.pl