Journal of Applied Genetics 51(2), 2010, pp. 123-131
Transferability, amplification quality, and genome specificity of microsatellites in Brassica carinata and related species
A. Marquez-Lema, L. Velasco, B. Perez-Vich
Abstract: No information is available on the transferability and amplification quality of microsatellite (SSR) markers of the public domain in Brassica carinata A. Braun. The objective of the presented research was to study the amplification of a set of 73 SSRs from B. nigra (L.) Koch and B. napus L. in B. carinata, and to compare the results with those obtained in the amplification of the same markers in other Brassica species of the U triangle. This set of SSRs from B. nigra (B genome) and B. napus (AC genome) allows the identification of the 3 basic genomes of the Brassica species tested. 94.3% of the SSR markers from B. nigra and 97.4% of those from B. napus amplified SSR-specific products in B. carinata. Very high-quality amplification with a strong signal and easy scoring in B. carinata was recorded for 52.8% of the specific loci from B. nigra SSRs and 59.3% of the specific loci from B. napus SSRs, compared to 66.7% in B. nigra and 62.8% in B. napus. Genome specificity and amplification quality of B. nigra and B. napus SSR markers in the 6 species under study is reported. High-quality transferable SSR markers provide an efficient and cost-effective platform to advance in molecular research in B. carinata.
Key words: Brassica carinata, Brassica napus, Brassica nigra, microsatellite markers, simple sequence repeats
Correspondence: B. Perez-Vich, Institute for Sustainable Agriculture (CSIC), Alameda del Obispo s/n, E-14004 Cordoba, Spain; e-mail: email@example.com
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