Journal of Applied Genetics 50(1), 2009, pp. 25-28

Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat-Secale africanum introgression lines

Juqing Jia,Zujun Yang,Guangrong Li, Cheng Liu, Mengping Lei, Tao Zhang, Jianping Zhou, Zhenglong Ren

Abstract: A repetitive sequence of 411 bp, named pSaO5411, was identified in the Secale africanum genome (Ra) by random amplified polymorphic DNA (RAPD) analysis of wheat and wheat-S. africanum amphiploids. GenBank BLAST search revealed that the sequence of pSaO5411 was highly homologous to a part of a Ty1-copia retrotransposon. Fluorescence in situ hybridization (FISH) analyses indicated that pSaO5411 was significantly hybridized to S. africanum chromosomes of a wheat-S. africanum amphiploid, and it was dispersed along the Secale chromosome arms except the terminal regions. Basing on the sequence of pSaO5411, a pair of sequence-characterized amplified region (SCAR) primers were designed, and the resultant SCAR marker was able to target both cultivated rye and the wild Secale species, which also enabled to identify effectively the S. africanum chromatin introduced into the wheat genome.

Key words: FISH, molecular marker, Secale africanum, Ty1-copia, retrotransposon.

Correspondence: Z. Yang, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; e-mail: yangzujun@uestc.edu.cn