Journal of Applied Genetics 49(4), 2008, pp. 313-320

Chromosomal localization of a novel repetitive sequence in the Chenopodium quinoa genome

Bozena Kolano, Andrzej Plucienniczak, Miroslaw Kwasniewski, Jolanta Maluszynska

Abstract: In this study, a novel repetitive sequence pTaq10 was isolated from the Taq I digest of the genomic DNA of the pseudocereal Chenopodium quinoa. Sequence analysis indicated that this 286-bp monomer is not homologous to any known retroelement sequence. FISH and Southern blot analysis showed that this sequence is characterized by an interspersed genomic organization. After FISH, hybridization signals were observed as small dots spread throughout all of the chromosomes. pTaq hybridization signals were excluded from 45S rRNA gene loci, but they partly overlapped with 5S rDNA loci. pTaq10 is not a species-specific sequence, as it was also detected in C. berlandieri.

Key words: Chenopodium, chromosomes, FISH, repetitive sequence.

Correspondence: B. Kolano, Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland; e-mail: bkolano@us.edu.pl