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Journal of Applied Genetics 48(3), 2007, pp. 233-239

Molecular analysis of a novel tandemly organized repetitive DNA sequence in Citrus limon (L.) Burm

Bruna De Felice, Loredana F. Ciarmiello, Robert R. Wilson, Clara Conicella


Abstract: Repetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization of the genome of interest. Here, we report the isolation and molecular analysis of a novel tandemly organized repetitive DNA sequence from the genome of Citrus limon. Digestion of C. limon DNA with Hinf I produced a prominent fragment of approximately 300 bp. Southern blotting revealed a ladder composed of DNA fragments that were multimers of the 300-bp Hinf I band. Thus, Hinf I digestion revealed a novel satellite, which we have called the C. limon satellite DNA 300 (CL300). Sequence analysis shows significant homology between a portion of the CL300 monomer and the transposase box of an En/Spm-like element. The CL300 satellite was also detected in grapefruit, sour orange, trifoliate orange and kumquat. These results suggest that the CL300 repeat is an ancient satellite, and we propose that a significant portion originated by amplification of a genomic region containing the En/Spm-like transposase element.

Key words: Citrus limon, En/Spm element, repeated sequences, transposon.

Correspondence: B. De Felice, Department of Life Sciences, University of Naples II, Via Vivaldi 43, 81100 Caserta, Italy; e-mail: bruna.defelice@unina2.it

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