Journal of Applied Genetics 48(1), 2007, pp. 69-71
Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle
Urszula Czarnik, Tadeusz Zabolewicz, Janusz Strychalski, Grzegorz Grzybowski, Marcin Bogusz, Krzysztof Walawski
Abstract: The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls in the 3 analysed polymorphic sites within the PRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows.
Key words: Polish Holstein-Friesian cattle, prion, PRNP, polymorphism, haplotypes.
Correspondence: U. Czarnik, Department of Animal Genetics, University of Warmia and Mazury, M. Oczapowskiego 5, 10-957 Olsztyn, Poland; e-mail:firstname.lastname@example.org
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