Journal of Applied Genetics 48(1), 2007, pp. 35-42
EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections
Han-Yan Wang, Yu-Ming Wei, Ze-Hong Yan, You-Liang Zheng
Abstract: SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which tri-nucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.
Key words: EST-SSR, genetic diversity, Triticum durum L.
Correspondence: Y.L. Zheng, Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan 625014, China; e-mail: email@example.com
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