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Journal of Applied Genetics 48(1), 2007, pp. 11-24

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 RXL10 intercross

Pawel Milczarski, Aneta Banek-Tabor, Karolina Lebiecka, Stefan Stojalowski, Beata Myskow, Piotr Masojc


Abstract: A new genetic map of rye, developed by using the 541 Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70-109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.

Key words: genetic linkage map, molecular markers, Secale cereale L., rye.

Correspondence: P. Masojc, Department of Genetics and Plant Breeding, University of Agriculture in Szczecin, Slowackiego 17, 71-434 Szczecin, Poland; e-mail: pmasojc@agro.ar.szczecin.pl

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