JOURNAL OF
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Journal of Applied Genetics 47(3), 2006, pp. 273-275

Application of multiplex FISH, CGH and MSSCP techniques for cytogenetic and molecular analysis of transitional cell carcinoma (TCC) cells in voided urine specimens

Maria Constantinou, Aleksandra Binka-Kowalska, Edyta Borkowska, Ewa Zajac, Pawel Jalmuzna, Jozef Matych, Agnieszka Nawrocka, Bogdan Kaluzewski


Abstract: Multiplex FISH (UroVysion), Comparative Genomic Hybridization (CGH), and Multitemperature Single-Strand Conformation Polymorphism (MSSCP) were applied for non-invasive diagnosis and prognosis of bladder cancer. The UroVysion test was positive in 80% of patients with pT1 and in 100% of patients with either pT2 or pT3 tumours. Tumours with pT3T4 stages were characterized by high numbers of chromosomal imbalances, detected by CGH. The mutation of the p53 gene was detected in 16% of patients, but only in those with pT2 or pT3 tumours.

Key words: bladder cancer, CGH, FISH, MSSCP, TCC.

Correspondence: M. Constantinou, Department of Medical Genetics, Medical University of Lodz, Sterlinga 1/3, 91-425 Lodz, Poland; e-mail: majcon@kardio-sterling.lodz.pl

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