Journal of Applied Genetics 47(3), 2006, pp. 243-245
Characterization of the CHORI-240 BAC clones containing the bovine CSN1S1, CSN2, STATH, CSN1S2 and CSN3 genes
Alexei A. Sazanov, Tadeusz Malewski, Stanislaw Kaminski, Lech Zwierzchowski
Abstract: Nineteen BAC clones were identified by hybridization of the bovine genomic BAC library CHORI-240 with mixed CSN1S1- and CSN3-specific probes. Two of the clones were shown to contain the genes CSN1S1, CSN1S2, CSN2, STATH and CSN3, and five were proved to include the genes CSN2, STATH, CSN1S2 and CSN3. These data showed that the BAC contig was established for the whole casein cluster, including all known five genes.
Key words: BAC-libraries, Bos taurus, contig, casein genes.
Correspondence: T. Malewski, Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland; e-mail: email@example.com
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