Journal of Applied Genetics 47(2), 2006, pp. 139-141
Associations between the prolactin receptor gene polymorphism and reproductive traits of boars
Marek Kmiec, Arkadiusz Terman
Abstract: The prolactin receptor gene (PRLR), located on chromosome 16 in pigs, is a candidate gene for reproductive traits. The experiment was aimed to detect the DNA mutations in this gene and to find probable relations between the genotype and some reproductive traits in boars. The polymorphism in the PRLR gene was identified by PCR-RFLP method using specific primers and the restriction enzyme AluI. In total 229 boars of various breeds were genotyped. The frequency of allele A was estimated at 0.62 and allele B at 0.38. Genotype AA was found at a frequency of 0.45, AB at 0.35 and BB at 0.20. We found associations between PRLR genotype and ejaculate volume, sperm concentration, percentage of live sperm, and number of live sperm in the ejaculate (P < 0.01).
Key words: PCR-RFLP, prolactin receptor gene, Sus scrofa, reproduction.
Correspondence: M. Kmiec, Agricultural University of Szczecin, Department of Genetics and Animal Breeding, Doktora Judyma 6, 71-466 Szczecin, Poland; e-mail: M.Kmiec@biot.ar.szczecin.pl
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