Journal of Applied Genetics 46(4), 2005, pp. 375-379
Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis
Suman Sud, Navtej Singh Bains, Govinder Singh Nanda
Abstract: Genetic relationships among 20 elite wheat genotypes were studied using microsatellite markers and pedigree analysis. A total of 93 polymorphic bands were obtained with 25 microsatellite primer pairs. Coefficient of parentage (COP) values were calculated using parentage information at the expansion level of 5. The pedigree-based similarity (mean 0.115, range 0.00-0.53) was lower than the similarity assessed using microsatellite markers (mean 0.70, range 0.47-0.91). Similarity estimates were used to construct dendrograms by using the unweighted pair-group method with arithmetic averages (UPGMA). Clustering of genotypes in respect of marker-based similarity revealed two groups. Genotype PBW442 diverged and appeared as distinct from all other genotypes in both marker-based and pedigree-based analysis. The correlation of COP values with genetic similarity values based on microsatellite markers is low (r = 0.285, p < 0.05). The results indicate a need to develop wheat varieties with a diverse genetic background and to incorporate new variability into the existing wheat gene pool.
Key words: bread wheat, coefficient of parentage, genetic similarity, microsatellite markers, Triticum aestivum L.
Correspondence: S. Sud, Lab No. 2 49S, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Station, Trombay, Mumbai-40085, e-mail: Sood004@yahoo.co.in, Suman_sood2@yahoo.co.in
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