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Journal of Applied Genetics 46(4), 2005, pp. 371-373

Rye SCAR markers for male fertility restoration in the P cytoplasm are also applicable to marker-assisted selection in the C cytoplasm

Stefan Stojalowski, Miloslawa Jaciubek, Piotr Masojc

Abstract: The study aimed at testing the usefulness of recently developed SCAR markers on rye (Secale cereale L.) chromosome 4R in hybrid breeding based on the C source of male sterility-inducing cytoplasm. Of 10 markers studied, 4 revealed polymorphisms between 2 inbred lines (544cms-C and Ot0-20) crossed to develop F2 and BC1 mapping populations. Analyses performed on 94 F2 and 93 BC1 plants allowed to extend a formerly constructed genetic map of chromosome arm 4RL. Three SCAR markers (SCP14M55, SCP15M55 and SCP16M58) were mapped in the vicinity of gene Rfc1, which restores male fertility in the C cytoplasm. The 3 tested SCAR markers proved to be effective in marker-assisted selection (MAS) for male fertility/sterility.

Key words: cytoplasmic male sterility, SCAR markers, Secale cereale.

Correspondence: S. Stojalowski, Department of Genetics and Plant Breeding, Agricultural University, Slowackiego 17, 71-437 Szczecin, Poland, e-mail:

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