Journal of Applied Genetics 45(4), 2004, pp. 453-455

New polymorphism of FASN gene in chicken

Graciela Marrube, Felisa Rozen, Gabriel Bernardo Pinto, Natalia Pacienza, Julian Enrique Melo, Miguel Javier Huguet, Zulma Canet, Ruben Zandomeni, Maria Cristina Miquel

Abstract: Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.

Key words: Gallus gallus, Fatty-acid synthase.

Correspondence: G. Marrube, Genetics Area, Animal Production Department, Veterinary School, University of Buenos Aires, Chorroarin 280, 1427, Buenos Aires, Argentina; e-mail: gmarrube@fvet.uba.ar