Journal of Applied Genetics 45(3), 2004, pp. 321-324

Isozyme polymorphism and genetic structure of the population of Sorbus torminalis (L.) Crantz from the Bytyń Forest (Poland)

Leszek Bednorz, Lukasz Myczko, Piotr Kosinski

Abstract: Dormant buds collected from 35 wild service trees (Sorbus torminalis) in the Bytyń Forest were tested with horizontal gel electrophoresis to assess the genetic structure of the population. Among 16 investigated isozyme loci, seven loci (ADH-A, 6PGD-A, GDH-B, ME-A, SOD-A, PGM-A, PGM-B) proved to be polymorphic, whereas the other nine loci (SDH-A, SDH-B, DIA-C, DIA-D, FLE-A, FLE-B, GOT-B, IDH-A, IDH-B) were monomorphic. The number of alleles per polymorphic locus ranged from two to three, with a mean of 2.29. The average observed and expected heterozygosities were 0.2665 and 0.3462, respectively. The combined FIS value over all polymorphic loci was 0.2179, which reflects a substantial deficit of heterozygotes. Two polymorphic loci (SOD-A, PGM-A) were identified in S. torminalis for the first time.

Key words: electrophoresis, genetic structure, isozymes, Sorbus torminalis.

Correspondence: L Bednorz, Department of Botany, August Cieszkowski Agricultural University, Wojska Polskiego 71c, 60-625 Poznan, Poland; e-mail: lbednorz@owl.au.poznan.pl