Journal of Applied Genetics 45(2), 2004, pp. 189-193

Localisation of rDNA in the Lupinus genome during the cell cycle

Barbara NAGANOWSKA, Anna ZIELINSKA

Abstract: Observations of a specific rDNA locus behaviour during the cell cycle were made by fluorescent in situ hybridisation (FISH) in 12 Lupinus species. Due to the pattern of chromatin de-condensation in that locus, the number of relevant sites in interphase nuclei was twice as high as the number of signals on metaphase chromosomes. The description of successive phases and an attempt of an explanation are given.

Key words: cell cycle, fluorescent in situ hybridisation, Lupinus, 18S-25S rDNA.

Correspondence: B. NAGANOWSKA, Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszynska 34, 60-479 Poznan, Poland; e-mail: bnag@igr.poznan.pl