JOURNAL OF
APPLIED GENETICS
        
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Journal of Applied Genetics 45(1), 2004, pp. 73-76

Silent point mutation polymorphism of the bovine CD18 encoding gene

Urszula CZARNIK, Tadeusz ZABOLEWICZ, Marcin GALINSKI, Chandra S. PAREEK, Krzysztof WALAWSKI


Abstract: We report on a PCR-RFLP procedure for recognising of a silent point mutation of ITGB2 CD18 subunit gene in cattle. Polymorphism screening was performed in a Polish Black-and-White cattle population (n=210). The genotype and allele frequecies were established in the sires and cows. Further research is needed to explain the possible applications of the CD18 silent point mutation as a potential molecular marker for high milk productivity.

Key words: cattle, CD18 gene, silent point mutation, DNA polymorphism.

Correspondence: K. WALAWSKI, Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 5, 10-719 Olsztyn, Poland; e-mail: Walawski@uwm.edu.pl

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