JOURNAL OF
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Journal of Applied Genetics 44(3), 2003, pp. 413-418

The influence of calyculin A on lymphocytes in vitro

Agata KOWALSKA, Malgorzata SREBNIAK, Angelika WAWRZKIEWICZ, Kazimierz KAMINSKI


Abstract: Human lymphocytes were cultured in vitro and treated with calyculin A. The aim of this work was to estimate the influence of calyculin A on chromosome morphology and banding patterns. It was also interesting whether calyculin A treatment is useful in cytogenetic analysis of human karyotype. We proved that calyculin A induces chromosome condensation in lymphocytes and raises the mitotic index significantly. Moreover, calyculin A does not influence the banding patterns. Therefore it is concluded that calyculin A can be clinically useful for human karyotyping.

Key words: calyculin A, human karyotype, lymphocyte cell culture.

Correspondence: M. SREBNIAK, Department of Perinatology and Gynaecology, Silesian Medical Academy, Plac Traugutta 6, 41-800 Zabrze, Poland, e-mail: srebna@poczta.onet.pl

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