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Journal of Applied Genetics 44(3), 2003, pp. 379-382

Detection of the XXY trisomy in a bull by using sex chromosome painting probes

Ewa SLOTA, Anna KOZUBSKA-SOBOCINSKA, Mariusz KOSCIELNY, Barbara DANIELAK-CZECH, Barbara REJDUCH


Abstract: Two cattle chromosome painting probes, identifying X and Y heterosomes, were applied to verify the diagnosis of XXY trisomy in an 8-month-old bull of the Polish Red breed. The probes were obtained after chromosome microdissection and labelled with biotin-16-dUTP. In all metaphase spreads, three fluorescence signals were observed - two X and one Y - confirming the diagnosis of a pure XXY trisomy.

Key words: cattle, FISH, painting probe, XXY trisomy.

Correspondence: E. SLOTA, Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, ul. Krakowska 1, 32-083 Balice, Poland, e-mail: eslota@izoo.krakow.pl

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