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Journal of Applied Genetics 44(3), 2003, pp. 355-360

Assessment of RAPD markers for barley doubled haploid lines resistant and susceptible to Fusarium culmorum at seedling and adult plant growth stages

Jan BOCIANOWSKI, Jerzy CHELKOWSKI, Anetta KUCZYNSKA, Halina WISNIEWSKA, Maria SURMA, Tadeusz ADAMSKI


Abstract: Thirty doubled haploid (DH) lines of barley derived from F1 of a cross between the six-rowed cultivar Pomo and two-rowed cultivar Maresi were examined for susceptibility to Fusarium seedling blight (SB) and head blight (FHB), measured by mycotoxin (nivalenol) content of kernels. RAPD (random amplified polymorphic DNA) polymorphism was analysed by using 53 decamer primers. Amplification products (APs) were 200 bp up to 2000 bp in size on average 5.7 per primer and the total number of APs was 284, 51.06% of which were polymorphic. Only 32 APs differentiated the examined DH lines – 19 APs for nivalenol content of kernels and 13 for seedling resistance. DH lines segregated with continuous distribution of resistance to FHB and SB. At the seedling stage all DH lines exhibited lower susceptibility than parental cultivars, but in the adult stage only two lines (MP 2 and MP 7) appeared to be more resistant to FHB, i.e. accumulated in kernels a lower amount of mycotoxin than cultivars Maresi and Pomo.

Key words: barley, doubled haploids, Fusarium culmorum, nivalenol, scab.

Correspondence: J. Bocianowski, Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszynska 34, 60479 Poznan, Poland, e-mail: jboc@igr.poznan.pl

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