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Journal of Applied Genetics 44(2), 2003, pp. 185-189

Polymerase chain reaction-heteroduplex (PCR-HD) polymorphism within the bovine STAT5A gene

Krzysztof FLISIKOWSKI, Lech ZWIERZCHOWSKI


Abstract: Signal transducers and activators of transcription (STATs) are transcription factors mediating signals of various hormones and cytokines. STAT5A, previously known as the mammary gland factor (MGF), mediates the action of prolactin on milk protein gene expression in mammary epithelial cells. We used polymerase chain reaction-heteroduplex (PCR-HD) and sequencing methods for detection of nucleotide sequence polymorphism in intron 15 of the bovine STAT5A gene. A 281-bp gene fragment, from nt 12525 to nt 12806 (GenBank AJ 2

Key words: bovine, DNA sequencing, gene polymorphism, PCR-HD, STAT5A.

Correspondence: L. ZWIERZCHOWSKI, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland. e-mail: l.zwierzchowski@ighz.pl

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