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Journal of Applied Genetics 44(2), 2003, pp. 139-149

Leaf rust resistance genes of wheat: identification in cultivars and resistance sources

Lukasz STEPIEN, Lidia GOLKA, Jerzy CHELKOWSKI


Abstract: Thirty-seven wheat cultivars originating from seven European countries were examined by using sequence tagged site (STS) markers for seven Lr (leaf rust = brown rust) resistance genes against the fungal pathogen of wheat Puccinia recondita f. sp. tritici (Lr9, Lr10, Lr19, Lr24, Lr26 and Lr37). Additionally, 22 accessions with various Lr genes from two germplasm collections were tested. A Scar (sequence-characterized amplified region) marker for Lr24 and a CAPS (Cleaved Amplified Polymorphic Sequence) marker for Lr47 were also used to identify those genes in the wheat accessions. Each marker amplified one specific DNA fragment. Three Lr gene markers were identified in wheat cultivars (Lr10, Lr26 and Lr37). Another four markers (Lr9, Lr19, Lr24 and Lr47) were found in breeding lines carrying leaf rust resistance genes. The results were compared with leaf rust resistance gene postulations made in previous studies, based on multipathotype testing. Markers for Lr10, Lr26 and Lr37 may be useful in marker-assisted breeding.

Key words: leaf rust, resistance genes, STS markers, wheat.

Correspondence: J. CHELKOWSKI, Institute of Plant Genetics, Polish Academy of Science, ul. Strzeszynska 34. 60-479 Poznan, Poland, e-mail: jche@igr.poznan.pl

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