Journal of Applied Genetics 43(4), 2002, pp. 501-504

PCR-RFLPs within the lactate dehydrogenase (LDH-A) gene of the domestic pigeon (Columba livia var. domestica)

Andrzej DYBUS, Marek KMIEC

Abstract: A total of 45 racing pigeons were genotyped using PCR-RFLP method. PCR product of the LDH-A gene was amplified according to the Long-PCR procedure. The amplification products were digested with restriction enzymes. PCR-RFLPs for two restriction enzymes, HaeIII and NlaIV, were observed. Two pairs of alleles LDH-AA and LDH-AB for LDH-A-NlaIV polymorphism and LDH-AC and LDH-AD for LDH-A-HaeIII polymorphism were detected in the homozygous and heterozygous states. Frequencies of alleles were as follows: A – 0.622, B – 0.378 and C – 0.256, D – 0.744.

Key words: Columba livia, LDH-A gene, PCR-RFLP, racing pigeon.

Correspondence: A. DYBUS, Department of Genetics and Animal Breeding, Agricultural University of Szczecin, ul. Doktora Judyma 6, 71-460 Szczecin, Poland, e-mail: a.dybus@biot.ar.szczecin.pl