Journal of Applied Genetics 43(1), 2002, pp. 93-96
Distribution of polymorphic forms at the porcine GH locus in a population of day-10 pig embryos
Dorota LECHNIAK, Susan E. LONG, Anna K. NISSEN
Abstract: The present study describes an analysis of genotype and allele distribution at the porcine GH locus among day-10 pig embryos. Embryos were collected post mortem from 6 crossbred (Danish Landrace × Yorkshire) sows inseminated with mixed Duroc semen and individually frozen for later analysis. After extraction, DNA was subjected to PCR amplification and restriction analysis with Msp I and Hae II enzymes. The genotype frequencies were: Msp I CD 0.17, DD 0.83; and Hae II AA 0.33, AB 0.58; and BB 0.09. The Msp I CC genotype was not found among analysed embryos. To our knowledge, this is the first report on the genotype and allele distribution at the GH locus among early pig embryos.
Key words: day-10 embryo, GH gene, pig, polymorphism.
Correspondence: D. Lechniak, Agricultural University of Poznan, Dept. Genetics and Animal Breeding, ul. Wolynska 33, 60-637 Poznan, e-mail: email@example.com
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