Journal of Applied Genetics 43(1), 2002, pp. 43-47
Genetic relationship between Polish and Chinese strains of the mushroom Agaricus bisporus (Lange) Sing., determined by the RAPD method
Miroslawa STANIASZEK, Waldemar MARCZEWSKI, Krystian SZUDYGA, Jedrzej MASZKIEWICZ, Andrzej CZAPLICKI, Guo QIAN
Abstract: The genetic relationship between twenty-six strains of Agaricus bisporus were analysed by the RAPD (random amplified polymorphic DNA) method. DNA amplification was performed with the use of twelve arbitrary 10-mer primers. Four primers, which gave polymorphic band patterns were chosen for RAPD analysis. In total, they gave 24 distinguishable bands, of which nine were polymorphic. The conducted research showed that there is a great genetic similarity among the examined strains. Low polymorphism of the strains may be a proof of a limited genetic pool used in the cultivation of those strains.
Key words: genetic diversity, mushroom strains, RAPD.
Correspondence: M. Staniaszek, Research Institute of Vegetable Crops, ul. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland, e-mail: email@example.com
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