Journal of Applied Genetics 43(1), 2002, pp. 19-31

Cytogenetic analysis of an asymmetric potato hybrid

Malgorzata SREBNIAK, Ole RASMUSSEN, Jolanta MALUSZYNSKA

Abstract: An asymmetric potato hybrid and its parental lines were cytogenetically examined. DAPI (4’-6-diamidino-2-phenylindole) staining was used to count chromosomes in all analysed lines and revealed the presence of minichromosomes in the hybrid genome. Fluorescent in situ hybridization (FISH) with rDNA sequence as a probe helped to determine the ploidy level of analysed lines and revealed that none of the minichromosomes contains rDNA repeats. CMA (chromomycin A3) band occurred to be a new chromosome marker that identifies potato chromosome No.1. It was possible to detect a deletion in one of four chromosomes No. 1 of the asymmetric potato hybrid. On the basis of these analyses a karyotype of the asymmetric hybrid was constructed.

Key words: asymmetric hybrid, DAPI/CMA3, FISH, minichromosomes, potato, rDNA, silver staining.

Correspondence: J. MALUSZYNSKA, Department of Plant Anatomy and Cytology, Silesian University, ul. Jagiellonska 28, 40-032 Katowice, Poland, e-mail: maluszyn@us.edu.pl