JOURNAL OF
APPLIED GENETICS
        
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Journal of Applied Genetics 42(4), 2001, pp. 547-552

Molecular follow up of donor lymphocyte infusion in CML children after allogeneic bone marrow transplantation

Dariusz LADON, Anna PIECZONKA, Justyna JOLKOWSKA, Jacek WACHOWIAK, Michal WITT


Abstract: In this study we present the applicability of fluorescence in situ hybridisation (FISH) and RT-PCR to detect the minimal residual disease (MRD) in relapsed Ph+ children after donor lymphocyte infusion (DLI) post bone marrow transplantation (BMT). In both patients BCR/ABL fusion was detected and its transcript at the moment of relapse. After the DLI treatment in short time intervals a decreasing number of cells with BCR/ABL fusion were noticed and the expression of the hybrid gene disappeared. These results demonstrate that all the methods presented in this study provide a feasible, rapid and accurate way for the detecting of the minimal residual disease after DLI in Ph positive CML patients.

Key words: bone marrow transplantation, donor lymphocyte infusion, fluorescence in situ hybridisation, minimal residual disease, RT-PCR.

Correspondence: D. LADON, Institute of Human Genetics, Polish Academy of Sciences, ul. Strzeszynska 32, 60-479 Poznan, Poland.