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Journal of Applied Genetics 42(4), 2001, pp. 413-423

Powdery mildew resistance genes in wheat: verification of STS markers

Lukasz STEPIEN, Yu CHEN, Jerzy CHELKOWSKI, Krzysztof KOWALCZYK


Abstract: Five accessions of Aegilops speltoides and 67 European wheat cultivars (winter and spring) originating from the Czech Republic, Germany, Poland, Russia, Slovakia, United Kingdom, and 4 non-European wheat cultivars from Brazil and the USA were examined with molecular Sequence Tagged Site (STS) markers for resistance genes to powdery mildew: Pm 1, Pm 2, Pm 3 and Pm 13. All markers gave clear, repeatable results, although three of them (Pm 1, Pm 2 and Pm 3) appeared as not specific for resistance genes. Comparison of STS analysis results with Pm genes, postulated as the reaction type after inoculation with differential isolates of Erysiphe graminis f.sp. tritici (Blumeria graminis), revealed a high number of disparities. The marker for Pm 13 was not detected in any examined cultivar but was present in five accessions of Aegilops speltoides.

Key words: powdery mildew, resistance genes, STS markers, wheat.

Correspondence: J. CHELKOWSKI, Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszynska 34, 60-479 Poznan, Poland, e-mail: jche@igr.poznan.pl

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