Journal of Applied Genetics 42(2), 2001, pp. 153-176
The possible role of cell cycle stage in mammalian cloning
Jacek A. MODLINSKI, Joanna GRABAREK, Berenika PLUSA, Jolanta KARASIEWICZ
Abstract: Progress in mammalian cloning started from cloning embryos (of mice, rats, rabbits, sheep, goats, pigs, cattle and rhesus monkeys) and culminated in obtaining clones of sheep, cattle, pigs and mice from adult somatic cells. Knowing the relationship between the cell cycles of the recipient and the donor of cell nucleus in embryonic cloning by nuclear transfer one can adjust the phases of the cell cycle properly. Metaphase II recipients accept G1 (in most species) or G2 donors (in the mouse). Interphase recipients can harbour nuclei in all stages of cell cycle. Relatively little is known about somatic cloning. Two attitudes are applied: either the donor is in the G0 phase or the recipient is in a prolonged MII phase.
Key words: mammalian cloning, cloning embryos, cell cycles.
Correspondence: J.A. Modlinski, Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland.