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Journal of Applied Genetics 42(2), 2001, pp. 139-144

Inter-simple sequence repeat (ISSR) markers for the Ns resistance gene in potato (Solanum tuberosum L


Abstract: Inter-simple sequence repeat (ISSR) polymorphism was used for finding markers linked to the Ns gene, responsible for a resistance of potato (Solanum tuberosum L.) to potato virus S (PVS). The ISSR markers UBC811660 and UBC811950 were found to be linked to Ns. Linkage distances were estimated to be 2.6 cM and 6.6 cM, respectively. UBC811660 showed high accuracy for detection of PVS resistance in diploid potato clones. In tetraploids, among seventeen studied genotypes containing the resistance gene, this marker was revealed in eleven. UBC811660 can be a powerful tool for detection of genotypes carrying the Ns gene in diploid potato breeding programmes.

Key words: Ns gene, potato, ISSR markers, potato virus S, resistance.

Correspondence: W. Marczewski, Plant Breeding and Acclimatization Institute, 05-831 Mlochow, Poland, e-mail: